Problems involving biofouling processes are often observed in various materials used by industry, especially in shipbuilding, aviation and aerospace. Damages in equipment due to biocorrosion mechanisms caused by biofilms, product contamination, energy losses associated with the increased friction, increased resistance to heat transfer are some of the effects of microbial biofilms accumulation and represent significant losses to the global industry [1-2]. In general, it is estimated that annual spending due to biofouling are around seven billion dollars, including prevention, maintenance and fuel consumption [4-5]. Currently, there is a great appeal for the development of solutions to this problem with minimal damage to the environment. Recent research points titanium dioxide as responsible for the reduction or control growth of microorganisms. Such materials, when exposed to UV light, degrade organic material presenting antibacterial characteristics [2-3]. Face to the importance of the issue worldwide, this work studied biofouling in metal plates coated with paints incorporated with TiO₂ particles at different concentrations. Preliminary results have shown that titanium dioxide content influences the surface energy of the coated substrate. This result suggests that the adhesion of microorganisms can be affected due to the embedded TiO₂ content. Moreover, due to the photocatalytic activity of titanium dioxide, when activated with UV radiation, there is a change in the surface characteristics of the coating, changing its wettability, which may suggest a change in the adhesion of microorganisms depending on the TiO₂ content in paint. In addition it was evaluated the surface roughness and morphological characteristics of the material by SEM and confocal optical microscopy. Results show that the influence of the content of titanium dioxide added in the surface roughness. The adhesion of microorganisms which can be fixed by mechanical anchorage is dependent on the surface roughness